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The glutathione redox ratio (GSH: GSSG) is an indicator of overall glutathione status and intracellular reducing environment

Nakamura T, Prikhodko OA, Pirie E, Nagar S, Akhtar MW, Oh C-K, et al

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l-citrulline can reduce hydroxyl radical formation, independent of NO, by directly interacting with hydroxyl radicals via the alpha-amino acids in its protonated NH 3 state leading to water formation [67,68]

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Briefly, 22 l concentrated HCl (11.6 mol/L) was added to 100 l cell lysate (~ 500 g total protein)